2020

Comparative transcriptomic analysis reveals conserved transcriptional programs underpinning organogenesis and reproduction in land plants

Irene JulcaCamilla FerrariMaría Flores-TorneroSebastian ProostAnn-Cathrin LindnerDieter HackenbergLenka SteinbachováChristos MichaelidisSónia Gomes PereiraChandra Shekhar MisraTomokazu KawashimaMichael BorgFrédéric BergerJacob GoldbergMark JohnsonDavid HonysDavid TwellStefanie SprunckThomas DresselhausJörg D. BeckerMarek Mutwil

bioRxiv (also submitted to a journal for review) 

ARP2/3-independent WAVE/SCAR pathway and class XI myosin control sperm nuclear migration in flowering plants

Mohammad Foteh AliUmma FatemaXiongbo PengSamuel W. HackerDaisuke MaruyamaMeng-Xiang Sun, Tomokazu Kawashima

Proceedings of the National Academy of Sciences of the United States of America (2020)

Cellular dynamics of double fertilization and early embryogenesis in flowering plants

Ji Min Shin, Ling Yuan, Masaru Ohme-Takagi & Tomokazu Kawashima  

JEZ-B Molecular and Developmental Evolution (2020)

Plasmogamic paternal contributions to early zygotic development in flowering plants

Yukinosuke Ohnishi & Tomokazu Kawashima  

Frontiers in Plant Science (2020)

Confocal microscopy provides visual evidence and confirms the feasibility of dsRNA delivery to emerald ash borer through plant tissues

Flavia Pampolini, Thais B. Rodrigues, Ramya S. Leelesh, Tomokazu Kawashima & Lynne K. Rieske 

Journal of Pest Science (2020)

Targeted reprogramming of H3K27me3 resets epigenetic memory in plant paternal chromatin

Michael Borg, Yannick Jacob, Daichi Susaki, Chantal LeBlanc, Daniel Buendía, Elin Axelsson, Tomokazu Kawashima, Philipp Voigt, Leonor Boavida, Jörg Becker, Tetsuya Higashiyama, Robert Martienssen & Frédéric Berger

Nature Cell Biology (2020)

Male Chromatin needs to relax to get seeds started

Tomokazu Kawashima

Plant & Cell Physiology pcz211 (2019)

2019

Building New Insights in Plant Gametogenesis from an Evolutionary Perspective

Tetsuya Hisanaga, Shohei Yamaoka, Tomokazu Kawashima, Asuka Higo, Keiji Nakajima, Takashi Araki, Takayuki Kohchi, Frederic Berger

Nature Plants 5:663-669 (2019)

Gamete Nulcear Migration in Animals and Plants

Umma Fatema, Mohammad F. Ali, Zheng Hu, Anthony Clark, and Tomokazu Kawashima

Frontiers in Plant Science 10, Article 517 (2019)

2018

Transcription factor DUO1 generated by neo-functionalization is associated with evolution of sperm differentiation in plants

A Higo, T Kawashima, et al.,

Nature Communications 9: Article number 5283

Gifu 2018: meeting of the International Association of Sexual Plant Reproduction Research

T Kawashima, MA Johnson

Plant Reproduction 32:137-139 (2019)

A Shared cis-Regulatory Module Activates Transcription in the Suspensor of Plant Embryos

KF Henry, AQ Bui, T Kawashima, RB Goldberg

Proceedings of National Academy of Sciences 115: E5824-5833 (2018)

A pharmacological study of Arabidopsis cell fusion between the persistent synergid and endosperm

Kazuki Motomura, Tomokazu Kawashima, Frédéric Berger, Tetsu Kinoshita, Tetsuya Higashiyama, Daisuke Maruyama

Journal of Cell Sciences, jcs. 204123, 2018

2017

Insights into Land Plant Evolution Garnered from the Marchantia polymorpha Genome

JL Bowman, T Kohchi, KT Yamato, J Jenkins, S Shu, K Ishizaki, et. al.,
Cell 171 (2), 287-304. e15

Live-Cell Imaging of F-Actin Dynamics During Fertilization in Arabidopsis thaliana

D Susaki, D Maruyama, R Yelagandula, F Berger, T Kawashima
Methods in Molecular Biology, 47-54

Genome-Wide Profiling of Histone Modifications and Histone Variants in Arabidopsis thaliana and Marchantia polymorpha

Ramesh Yelagandula, Akihisa Osakabe, Elin Axelsson, Frederic Berger, Tomokazu Kawashima
Methods in Molecular Biology 1610, 93-106, 2017

2016

Cytoskeleton dynamics control the first asymmetric cell division in Arabidopsis zygote

Yusuke Kimata, Takumi Higaki, Tomokazu Kawashima, Daisuke Kurihara, Yoshikatsu Sato, Tomomi Yamada, Seiichiro Hasezawa, Frederic Berger, Tetsuya Higashiyama, Minako Ueda
Proceedings of National Academy of Sciences USA 113 (49), 14157–14162, 2016

Fertilization-independent cell-fusion between the synergid and central cell in the polycomb mutant

Kazuki Motomura, Frédéric Berger, Tomokazu Kawashima, Tetsu Kinoshita, Tetsuya Higashiyama, Daisuke Maruyama
Cell Structure and Function 41 (2), 121-125, 2016

2015

Selective nuclear elimination in multinucleate cells

Daisuke Maruyama, Tomokazu Kawashima, Tetsuya Higashiyama
Oncotarget 6 (31), 30447, 2015

Diversification of histone H2A variants during plant evolution

Tomokazu Kawashima, Zdravko J Lorković, Ryuichi Nishihama, Kimitsune Ishizaki, Elin Axelsson, Ramesh Yelagandula, Takayuki Kohchi, Frederic Berger
Trends in plant science 20 (7), 419-425, 2015

A cis-regulatory module activating transcription in the suspensor contains five cis-regulatory elements

Kelli F Henry, Tomokazu Kawashima, Robert B Goldberg
Plant molecular biology 88 (3), 207-217, 2015

The central cell nuclear position at the micropylar end is maintained by the balance of F-actin dynamics, but dispensable for karyogamy in Arabidopsis

Tomokazu Kawashima, Frederic Berger
Plant reproduction 28 (2), 103-110, 2015

Rapid elimination of the persistent synergid through a cell fusion mechanism

Daisuke Maruyama, Ronny Völz, Hidenori Takeuchi, Toshiyuki Mori, Tomoko Igawa, Daisuke Kurihara, Tomokazu Kawashima, Minako Ueda, Masaki Ito, Masaaki Umeda, Shuh-ichi Nishikawa, Rita Groß-Hardt, Tetsuya Higashiyama
Cell 161 (4), 907-918, 2015

2014

Dynamic F-actin movement is essential for fertilization in Arabidopsis thaliana

Tomokazu Kawashima, Daisuke Maruyama, Murat Shagirov, Jing Li, Yuki Hamamura, Ramesh Yelagandula, Yusuke Toyama, Frédéric Berger
eLife 3, e04501, 2014

Epigenetic reprogramming in plant sexual reproduction

Tomokazu Kawashima, Frédéric Berger
Nature Reviews Genetics 15 (9), 613-624, 2014

The histone variant H2A. W defines heterochromatin and promotes chromatin condensation in Arabidopsis

Ramesh Yelagandula, Hume Stroud, Sarah Holec, Keda Zhou, Suhua Feng, Xuehua Zhong, Uma M Muthurajan, Xin Nie, Tomokazu Kawashima, Martin Groth, Karolin Luger, Steven E Jacobsen, Frédéric Berger
Cell 158 (1), 98-109, 2014

2011

Green love talks; cell–cell communication during double fertilization in flowering plants

Tomokazu Kawashima, Frederic Berger
AoB Plants 2011, plr015, 2011

TOMO

2010

The suspensor: not just suspending the embryo

T Kawashima, RB Goldberg
Trends in plant science 15 (1), 23-30, 2010

TOMO

2009

Identification of cis-regulatory sequences that activate transcription in the suspensor of plant embryos

Tomokazu Kawashima, Xingjun Wang, Kelli F Henry, Yuping Bi, Koen Weterings, Robert B Goldberg
Proceedings of the National Academy of Sciences USA 106 (9), 3627-3632, 2009

TOMO

2007

Using genomics to study legume seed development

BH Le, JA Wagmaister, T Kawashima, AQ Bui, JJ Harada, RB Goldberg
Plant physiology 144 (2), 562-574, 2007